Acetylcholinesterase Antibody / ACHE

NSJ Bioreagents
Product Code: NSJ-F50830
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50830-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50830-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Acetylcholinesterase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Confocal immunofluorescent analysis of Acetylcholinesterase antibody with human NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments labeled with Alexa Fluor 555 Phalloidin (red). DAPI used as a nuclear counterstain (blue).
2 / 4
IHC analysis of FFPE human brain tissue stained with Acetylcholinesterase antibody
3 / 4
Acetylcholinesterase antibody flow cytometric analysis of human NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
Acetylcholinesterase antibody western blot analysis in human 1) Jurkat, 2) Raji and 3) Y79 lysate. Predicted molecular weight ~68 kDa with a possible ~58 kDa isoform.

Confocal immunofluorescent analysis of Acetylcholinesterase antibody with human NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments labeled with Alexa Fluor 555 Phalloidin (red). DAPI used as a nuclear counterstain (blue).
IHC analysis of FFPE human brain tissue stained with Acetylcholinesterase antibody
Acetylcholinesterase antibody flow cytometric analysis of human NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Acetylcholinesterase antibody western blot analysis in human 1) Jurkat, 2) Raji and 3) Y79 lysate. Predicted molecular weight ~68 kDa with a possible ~58 kDa isoform.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Description:
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 147-175 from the human protein was used as the immunogen for this Acetylcholinesterase antibody.
Limitation:
This Acetylcholinesterase antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Guinea pig
Uniprot #:
P22303