Bax Antibody

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NSJ Bioreagents
Product Code: NSJ-F40098
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40098-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40098-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Bax antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Bax antibody western blot analysis in HL-60 lysate
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IHC analysis of FFPE human hepatocarcinoma stained with Bax antibody
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Flow cytometric analysis of HepG2 cells using Bax antibody (green) compared to a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of Bax antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the Bax gene.
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Bax antibody western blot analysis in HL-60 lysate
IHC analysis of FFPE human hepatocarcinoma stained with Bax antibody
Flow cytometric analysis of HepG2 cells using Bax antibody (green) compared to a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of Bax antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the Bax gene.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the Bax antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 41-76 (BH3 domain) from the human protein was used as the immunogen for this Bax antibody.
Limitation:
This Bax antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine
Uniprot #:
Q07812