HuR Antibody

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NSJ Bioreagents
Product Code: NSJ-F51453
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51453-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51453-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the HuR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of HuR antibody and HeLa, Jurkat, CEM, Ramos lysate.
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IHC analysis of FFPE human brain tissue stained with HuR antibody
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HuR antibody flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
Western blot analysis of HuR antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the ELAVL1 gene.
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Western blot analysis of HuR antibody and HeLa, Jurkat, CEM, Ramos lysate.
IHC analysis of FFPE human brain tissue stained with HuR antibody
HuR antibody flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of HuR antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the ELAVL1 gene.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the HuR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ELAVL1/HuR is involved in 3'-UTR ARE-mediated MYC stabilization. It binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This HuR antibody was produced from rabbits immunized with human ELAVL1/HuR recombinant protein.
Limitation:
This HuR antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q15717