IL-12 Antibody

NSJ Bioreagents
Product Code: NSJ-F51357
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51357-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51357-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the IL-12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of IL-12 antibody and MDA-MB231 lysate. Predicted molecular weight ~ 24 kDa.
2 / 3
IHC analysis of FFPE human breast carcinoma stained with IL-12 antibody
3 / 3
IL-12 antibody flow cytometry analysis of MDA-MB231 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of IL-12 antibody and MDA-MB231 lysate. Predicted molecular weight ~ 24 kDa.
IHC analysis of FFPE human breast carcinoma stained with IL-12 antibody
IL-12 antibody flow cytometry analysis of MDA-MB231 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the IL-12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 168-195 from the human protein was used as the immunogen for this IL-12 antibody.
Limitation:
This IL-12 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P29459