PCSK9 Antibody

NSJ Bioreagents
Product Code: NSJ-F50317
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50317-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50317-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PCSK9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from HeLa cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
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Western blot analysis of lysate from HepG2, HeLa, SW480 cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
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Western blot analysis of lysate from SW480 cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
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Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma section using PCSK9 antibody
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Flow cytometric analysis of HeLa cells using PCSK9 antibody (green) compared to an isotype control of rabbit IgG (blue); Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
6 / 6
Flow cytometric analysis of A431 cells using PCSK9 antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.

Western blot analysis of lysate from HeLa cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
Western blot analysis of lysate from HepG2, HeLa, SW480 cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
Western blot analysis of lysate from SW480 cell line using PCSK9 antibody. Predicted size: Pro/mature ~74/64 kDa
Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma section using PCSK9 antibody
Flow cytometric analysis of HeLa cells using PCSK9 antibody (green) compared to an isotype control of rabbit IgG (blue); Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
Flow cytometric analysis of A431 cells using PCSK9 antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.

Further Information

Application Details :
Flow Cytometry: 1:25,IHC (Paraffin): 1:25,Western blot: 1:1000
Application Note:
Titration of the PCSK9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 144-173 from the human protein was used as the immunogen for this PCSK9 antibody.
Limitation:
This PCSK9 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q8NBP7