FGFR4 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50622
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50622-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50622-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the FGFR4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
FGFR4 antibody western blot analysis in 293 lysate. Observed molecular weight: 88~125 kDa depending on phosphorylation and glycosylation level.
2 / 4
Fluorescent confocal image of HeLa cells stained with FGFR4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with primary Ab (1:200, 2 h at room temperature). For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of FGFR4 to the nucleus, supported by Human Protein Atlas Data (<a href=https://www.proteinatlas.org/ENSG00000160867)><a href=https://www.proteinatlas.org/ENSG00000160867><a href=https://www.proteinatlas.org/ENSG00000160867>https://www.proteinatlas.org/ENSG00000160867</a></a>).
3 / 4
Flow cytometric analysis of WiDr cells using FGFR4 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
IHC analysis of FFPE human hepatocarcinoma tissue stained with the FGFR4 antibody
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FGFR4 antibody western blot analysis in 293 lysate. Observed molecular weight: 88~125 kDa depending on phosphorylation and glycosylation level.
Fluorescent confocal image of HeLa cells stained with FGFR4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with primary Ab (1:200, 2 h at room temperature). For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of FGFR4 to the nucleus, supported by Human Protein Atlas Data (<a href=https://www.proteinatlas.org/ENSG00000160867)><a href=https://www.proteinatlas.org/ENSG00000160867><a href=https://www.proteinatlas.org/ENSG00000160867>https://www.proteinatlas.org/ENSG00000160867</a></a>).
Flow cytometric analysis of WiDr cells using FGFR4 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human hepatocarcinoma tissue stained with the FGFR4 antibody

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:200,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the FGFR4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FGFR4 is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR-4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR-4. Mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 24-55 from the human protein was used as the immunogen for this FGFR4 antibody.
Limitation:
This FGFR4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P22455