Anti-Vimentin Antibody

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NSJ Bioreagents
Product Code: NSJ-F48162
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48162-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48162-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the anti-Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Fluorescent confocal image of SY5Y cells stained with anti-Vimentin antibody at 1:100. Vimentin is localized to the cytoskeleton.
2 / 4
Western blot analysis of anti-Vimentin antibody and NCI-H460 lysate
3 / 4
Anti-Vimentin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue.
4 / 4
Anti-Vimentin antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Fluorescent confocal image of SY5Y cells stained with anti-Vimentin antibody at 1:100. Vimentin is localized to the cytoskeleton.
Western blot analysis of anti-Vimentin antibody and NCI-H460 lysate
Anti-Vimentin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue.
Anti-Vimentin antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the anti-Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 430-457 from the human protein was used as the immunogen for this anti-Vimentin antibody.
Limitation:
This anti-Vimentin antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Chicken, Hamster, Mouse, Pig, Primate, Rat, Xenopus
Uniprot #:
P08670