GABARAP Antibody

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NSJ Bioreagents
Product Code: NSJ-F46304
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F46304-0.08ML0.08 ml£260.00
Quantity:
NSJ-F46304-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the GABARAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of GABARAP antibody and Hela cells treated with 26S proteasome complex blocker MG132.
2 / 5
Western blot analysis of GABARAP antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (2).
3 / 5
Fluorescent image of Chloroquine treated U251 cells stained with GABARAP antibody. Alexa Fluor 488 secondary (green) was used. Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
4 / 5
GABARAP antibody testing of mouse MEFs and mouse motor neurons. Antibodies used were GABARAP antibody (AF488 conjugated) and anti-Tuj1 (AF647 conjugated).
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IHC analysis of FFPE human hepatocarcinoma tissue stained with GABARAP antibody
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Western blot testing of GABARAP antibody and Hela cells treated with 26S proteasome complex blocker MG132.
Western blot analysis of GABARAP antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (2).
Fluorescent image of Chloroquine treated U251 cells stained with GABARAP antibody. Alexa Fluor 488 secondary (green) was used. Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
GABARAP antibody testing of mouse MEFs and mouse motor neurons. Antibodies used were GABARAP antibody (AF488 conjugated) and anti-Tuj1 (AF647 conjugated).
IHC analysis of FFPE human hepatocarcinoma tissue stained with GABARAP antibody

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the GABARAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for this GABARAP antibody.
Limitation:
This GABARAP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Rabbit
Uniprot #:
O95166