PDX1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F47892
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F47892-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47892-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PDX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Fluorescent confocal image of SY5Y cells stained with PDX1 antibody. Alexa Fluor 488 conjugated secondary (green) was used. Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDX1 immunosignal to the nucleus.
2 / 4
Western blot analysis of PDX1 antibody and NCI-H460 lysate
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Western blot analysis of PDX1 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (2).
4 / 4
IHC analysis of FFPE human hepatocarcinoma tissue stained with PDX1 antibody
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Fluorescent confocal image of SY5Y cells stained with PDX1 antibody. Alexa Fluor 488 conjugated secondary (green) was used. Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDX1 immunosignal to the nucleus.
Western blot analysis of PDX1 antibody and NCI-H460 lysate
Western blot analysis of PDX1 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (2).
IHC analysis of FFPE human hepatocarcinoma tissue stained with PDX1 antibody

Documents

Further Information

Application Details :
Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the PDX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for this PDX1 antibody.
Limitation:
This PDX1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
O00330