PSMA Antibody

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NSJ Bioreagents
Product Code: NSJ-F43183
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F43183-0.08ML0.08 ml£260.00
Quantity:
NSJ-F43183-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PSMA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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PSMA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma.
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PSMA antibody western blot analysis in ZR-75-1 lysate. Observed molecular weight ~ 100 kDa.
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Confocal immunofluorescent analysis of PSMA antibody with ZR-75-1 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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PSMA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma.
PSMA antibody western blot analysis in ZR-75-1 lysate. Observed molecular weight ~ 100 kDa.
Confocal immunofluorescent analysis of PSMA antibody with ZR-75-1 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the PSMA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GCPII is a zinc metalloenzyme that resides in membranes. Most of the enzyme resides in the extracellular space. GCPII is a class II membrane glycoprotein. It catalyzes the hydrolysis of N-acetylaspartylglutamate (NAAG) to glutamate and N-acetylaspartate (NAA). Neuroscientists primarily use the term NAALADase in their studies, while those studying folate metabolism use folate hydrolase, and those studying prostate cancer or oncology, PSMA. All of which refer to the same protein, glutamate carboxypeptidase II. PSMA is strongly expressed in the human prostate, being a hundredfold greater than the expression in most other tissues. In cancer, it is upregulated in expression and has been called the second-most-upregulated gene in prostate cancer, with increase of 8- to 12-fold over the noncancerous prostate. In human prostate cancer, the higher expressing tumors are associated with quicker time to progression and a greater percentage of patients suffering relapse. [Wiki]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 161-190 from the human protein was used as the immunogen for this PSMA antibody.
Limitation:
This PSMA antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Pig
Uniprot #:
Q04609