Ubiquitin Antibody

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NSJ Bioreagents
Product Code: NSJ-F42064
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42064-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42064-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Ubiquitin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of Ubiquitin antibody and HeLa cell lysate
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IHC analysis of FFPE human hepatocarcinoma stained with the Ubiquitin antibody
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Characterization of FOXP2 isoforms using FOXP2 Ab (green) and Ubiquitin antibody (aggresome marker) (red). Nuclei are marked by DAPI staining (blue). Ubiquitin co-localizes with FOXP2.10t aggregates suggesting that these cellular bodies represent aggresomes.
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Western blot analysis of Ubiquitin antibody and HeLa cell lysate
IHC analysis of FFPE human hepatocarcinoma stained with the Ubiquitin antibody
Characterization of FOXP2 isoforms using FOXP2 Ab (green) and Ubiquitin antibody (aggresome marker) (red). Nuclei are marked by DAPI staining (blue). Ubiquitin co-localizes with FOXP2.10t aggregates suggesting that these cellular bodies represent aggresomes.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes Ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 36-66 from the human protein was used as the immunogen for this Ubiquitin antibody.
Limitation:
This Ubiquitin antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, C. elegans, Chicken, Drosophila, Mouse, Pig, Primate, Rat, Yeast
Uniprot #:
P0CG48