ALDH1A1 Antibody

NSJ Bioreagents
Product Code: NSJ-F44050
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44050-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44050-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ALDH1A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from (1) HepG2 cell line and (2) human lung tissue lysate using ALDH1A1 antibody diluted at 1:1000.
2 / 4
Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody at 1:100. ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.
3 / 4
ALDH1A1 antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
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ALDH1A1 antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of lysate from (1) HepG2 cell line and (2) human lung tissue lysate using ALDH1A1 antibody diluted at 1:1000.
Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody at 1:100. ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.
ALDH1A1 antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
ALDH1A1 antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:100,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the ALDH1A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This ALDH1A1 antibody was produced from rabbits immunized with human ALDH1A1 recombinant protein.
Limitation:
This ALDH1A1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P00352