Parkin Antibody

NSJ Bioreagents
Product Code: NSJ-F49620
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49620-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49620-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Parkin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot analysis of lysate from (1) SH-SY5Y cell line and (2) human brain tissue lysate using Parkin antibody at 1:1000. Predicted molecular weight: 50-60 kDa.
2 / 4
Parkin antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 4
Confocal immunofluorescent analysis of Parkin antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
4 / 4
Parkin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.

Western blot analysis of lysate from (1) SH-SY5Y cell line and (2) human brain tissue lysate using Parkin antibody at 1:1000. Predicted molecular weight: 50-60 kDa.
Parkin antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of Parkin antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Parkin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.

Further Information

Application Details :
Western blot: 1:250-1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the Parkin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. The function of Park2 is not well-known; however, it may play a role in the ubiquitin-mediated proteolytic pathway. Mutations in this gene are known to cause autosomal recessive juvenile parkinsonism. Alternative splicing of this gene produces three known products of undetermined function.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 111-140 from the human protein was used as the immunogen for this Parkin antibody.
Limitation:
This Parkin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
O60260