Mib1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F47999
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F47999-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47999-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Mib1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Mib1 antibody western blot analysis in K562 lysate.
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Immunofluorescent staining of HeLa cells incubated with Mib1 antibody at a dilution of 1:20.
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IHC analysis of FFPE human hepatocarcinoma tissue stained with the Mib1 antibody
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Mib1 antibody western blot analysis in K562 lysate.
Immunofluorescent staining of HeLa cells incubated with Mib1 antibody at a dilution of 1:20.
IHC analysis of FFPE human hepatocarcinoma tissue stained with the Mib1 antibody

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:20-1:100,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the Mib1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 13-42 from the human protein was used as the immunogen for this Mib1 antibody.
Limitation:
This Mib1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Zebrafish, Xenopus
Uniprot #:
Q86YT6