HDAC9 Antibody

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NSJ Bioreagents
Product Code: NSJ-F41192
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41192-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41192-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot the HDAC9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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HDAC9 antibody NSJ# F41192 and <a href=../tds/hdac9-antibody-f41193>NSJ# F41193</a> were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Both antibodies detect the protein in transfected cells but not non-transfected.
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HDAC9 antibody NSJ# F41192 and <a href=../tds/hdac9-antibody-f41193>NSJ# F41193</a> can both immunoprecipitate the protein from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute)
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Immunoblots for MITR (HDAC9), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman, Upstate Medical University, New York.
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Immunofluorescence staining of MITR (HDAC9) for a compartmentalization study in undifferentiated transfected C2C12 myoblasts. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
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IHC analysis of FFPE human hepatocarcinoma stained with HDAC9 antibody
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HDAC9 antibody NSJ# F41192 and <a href=../tds/hdac9-antibody-f41193>NSJ# F41193</a> were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Both antibodies detect the protein in transfected cells but not non-transfected.
HDAC9 antibody NSJ# F41192 and <a href=../tds/hdac9-antibody-f41193>NSJ# F41193</a> can both immunoprecipitate the protein from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute)
Immunoblots for MITR (HDAC9), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman, Upstate Medical University, New York.
Immunofluorescence staining of MITR (HDAC9) for a compartmentalization study in undifferentiated transfected C2C12 myoblasts. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
IHC analysis of FFPE human hepatocarcinoma stained with HDAC9 antibody

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunoprecipitation: 1:100,Immunofluorescence: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the HDAC9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 2-32 from the human protein was used as the immunogen for this HDAC9 antibody.
Limitation:
This HDAC9 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Chicken
Uniprot #:
Q9UKV0