ErbB2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50600
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50600-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50600-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of ErbB2 in T47D cell lysate, either noninduced (Lane 1) or induced with HRG (2).
2 / 3
Flow cytometric analysis of MCF-7 cells using HER2 / ErbB2 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 3
IHC analysis of FFPE human breast carcinoma tissue stained with ErbB2 antibody
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Western blot analysis of ErbB2 in T47D cell lysate, either noninduced (Lane 1) or induced with HRG (2).
Flow cytometric analysis of MCF-7 cells using HER2 / ErbB2 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human breast carcinoma tissue stained with ErbB2 antibody

Documents

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the ErbB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An Interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 21-52 from the human protein was used as the immunogen for this ErbB2 antibody.
Limitation:
This ErbB2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
P04626