SPHK1 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F40149
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40149-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40149-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SPHK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot analysis of SPHK1 antibody and 293 lysate
2 / 4
SPHK1 antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle.
3 / 4
Western blot analysis of SPHK1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the SPHK1 gene.
4 / 4
SPHK1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
❮❯

Western blot analysis of SPHK1 antibody and 293 lysate
SPHK1 antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle.
Western blot analysis of SPHK1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the SPHK1 gene.
SPHK1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the SPHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 313-343 from the human protein was used as the immunogen for this SPHK1 antibody.
Limitation:
This SPHK1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q9NYA1