CD19 Antibody

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NSJ Bioreagents
Product Code: NSJ-F44318
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44318-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44318-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the CD19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot analysis of lysate from Ramos cell line using CD19 antibody at 1:500. Expected size is 60~100 kDa depending on glycosylation level.
2 / 6
CD19 antibody western blot analysis in Raji lysate. Expected size is 60~100 kDa depending on glycosylation level.
3 / 6
Western blot analysis of CD19 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (2). Expected size is 60~100 kDa depending on glycosylation level.
4 / 6
Immunofluorescence analysis of CD19 antibody with paraffin-embedded human lymph tissue. Primary Ab was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
5 / 6
Flow cytometric analysis of CEM cells using CD19 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
6 / 6
IHC analysis of FFPE human lymph stained with CD19 antibody
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Western blot analysis of lysate from Ramos cell line using CD19 antibody at 1:500. Expected size is 60~100 kDa depending on glycosylation level.
CD19 antibody western blot analysis in Raji lysate. Expected size is 60~100 kDa depending on glycosylation level.
Western blot analysis of CD19 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (2). Expected size is 60~100 kDa depending on glycosylation level.
Immunofluorescence analysis of CD19 antibody with paraffin-embedded human lymph tissue. Primary Ab was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
Flow cytometric analysis of CEM cells using CD19 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human lymph stained with CD19 antibody

Documents

Further Information

Application Details :
Western blot: 1:500-100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the CD19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 143-172 from the human protein was used as the immunogen for this CD19 antibody.
Limitation:
This CD19 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P15391