CD63 Antibody

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NSJ Bioreagents
Product Code: NSJ-F49127
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49127-0.05ML0.05 ml£260.00
Quantity:
NSJ-F49127-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of CD63 antibody at 1:2000 dilution. Lane 1: A2058 lysate; 2: A375 lysate; 3: THP-1 lysate; 4: U-87 MG lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
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Western blot testing of CD63 antibody at 1:1000 dilution + human plasma lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
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Western blot testing of CD63 antibody at 1:1000 dilution. Lane 1: A2058 lysate; 2: MDA-MB-453 lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
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Western blot testing of human spleen tissue lysate with CD63 antibody. Predicted molecular weight: 25-60 kDa depending on glycosylation level.
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FACS testing of fixed and permeabilized human HL-60 cells with CD63 antibody. Blue = isotype control, Green = CD63 antibody.
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IHC testing of FFPE human melanoma tissue with CD63 antibody at 1:25 dilution. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human skin with CD63 antibody at 1:25 dilution. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human tonsil tissue with CD63 antibody. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of human 1) HL-60, 2) U-251 MG, 3) THP-1, 4) A2058 and 5) A375 lysate with CD63 antibody. Predicted molecular weight: 25-60 kDa depending on glycosylation level.
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Western blot testing of CD63 antibody at 1:2000 dilution. Lane 1: A2058 lysate; 2: A375 lysate; 3: THP-1 lysate; 4: U-87 MG lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
Western blot testing of CD63 antibody at 1:1000 dilution + human plasma lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
Western blot testing of CD63 antibody at 1:1000 dilution. Lane 1: A2058 lysate; 2: MDA-MB-453 lysate; Predicted band size: 25-60 kDa depending on glycosylation level.
Western blot testing of human spleen tissue lysate with CD63 antibody. Predicted molecular weight: 25-60 kDa depending on glycosylation level.
FACS testing of fixed and permeabilized human HL-60 cells with CD63 antibody. Blue = isotype control, Green = CD63 antibody.
IHC testing of FFPE human melanoma tissue with CD63 antibody at 1:25 dilution. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human skin with CD63 antibody at 1:25 dilution. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human tonsil tissue with CD63 antibody. HIER: steam tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) HL-60, 2) U-251 MG, 3) THP-1, 4) A2058 and 5) A375 lysate with CD63 antibody. Predicted molecular weight: 25-60 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunohistochemistry (FFPE): 1:25,FACS: 1:25 per 1xmillion cells
Application Note:
Titration of the CD63 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 163-190 from the human protein was used as the immunogen for this CD63 antibody.
Limitation:
This CD63 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P08962