Anti-EGFR Antibody

NSJ Bioreagents
Product Code: NSJ-F49183
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49183-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49183-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the anti-EGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of lysate from A431, HUVEC, PC3 cell line (left to right) using anti-EGFR antibody diluted at 1:1000 for each lane.
2 / 3
Anti-EGFR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue.
3 / 3
Anti-EGFR antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of lysate from A431, HUVEC, PC3 cell line (left to right) using anti-EGFR antibody diluted at 1:1000 for each lane.
Anti-EGFR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue.
Anti-EGFR antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the anti-EGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1004-1033 from the human protein was used as the immunogen for this anti-EGFR antibody.
Limitation:
This anti-EGFR antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P00533