Caspase-9 Antibody

NSJ Bioreagents
Product Code: NSJ-F50929
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50929-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50929-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Caspase-9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of Caspase-9 antibody and Jurkat lysate
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IHC analysis of FFPE human hepatocarcinoma with Caspase-9 antibody
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Flow cytometric analysis of MDA-231 cells using Caspase-9 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of Caspase-9 antibody and Jurkat lysate
IHC analysis of FFPE human hepatocarcinoma with Caspase-9 antibody
Flow cytometric analysis of MDA-231 cells using Caspase-9 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the Caspase-9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This Caspase-9 antibody was produced from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S196 of human CASP9.
Limitation:
This Caspase-9 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P55211