CD8 Antibody

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NSJ Bioreagents
Product Code: NSJ-F43566
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F43566-0.08ML0.08 ml£260.00
Quantity:
NSJ-F43566-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the CD8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Flow cytometric analysis of NCI-H292 cells using CD8 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
2 / 3
IHC analysis of FFPE human lung carcinoma tissue stained with CD8A antibody
3 / 3
Western blot analysis of CD8 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD8A gene (2).
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Flow cytometric analysis of NCI-H292 cells using CD8 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human lung carcinoma tissue stained with CD8A antibody
Western blot analysis of CD8 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD8A gene (2).

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the CD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 150-180 from the human protein was used as the immunogen for this CD8 antibody.
Limitation:
This CD8 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P01732