HLA-G Antibody

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NSJ Bioreagents
Product Code: NSJ-F51441
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51441-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51441-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the HLA-G antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of HLA-G antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the HLA-G gene.
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Western blot analysis of HLA-G antibody and NCI-H460 lysate. Predicted molecular weight ~40 kDa.
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Western blot analysis of lysate from human placenta tissue lysate using HLA-G antibody diluted at 1:1000.
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IHC analysis of FFPE human lung carcinoma stained with HLA-G antibody
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HLA-G antibody flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IHC analysis of FFPE human placenta stained with HLA-G antibody. HIER: boil tissue sections in pH 9 EDTA buffer for 20 min and allow to cool before testing.
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Western blot analysis of HLA-G antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the HLA-G gene.
Western blot analysis of HLA-G antibody and NCI-H460 lysate. Predicted molecular weight ~40 kDa.
Western blot analysis of lysate from human placenta tissue lysate using HLA-G antibody diluted at 1:1000.
IHC analysis of FFPE human lung carcinoma stained with HLA-G antibody
HLA-G antibody flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human placenta stained with HLA-G antibody. HIER: boil tissue sections in pH 9 EDTA buffer for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the HLA-G antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 62-89 from the human protein was used as the immunogen for this HLA-G antibody.
Limitation:
This HLA-G antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P17693