PHGDH Antibody

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NSJ Bioreagents
Product Code: NSJ-F48339
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48339-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48339-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PHGDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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PHGDH antibody western blot analysis in Ramos lysate. Predicted molecular weight ~56 kDa.
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PHGDH antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
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PHGDH antibody flow cytometric analysis of Ramos cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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PHGDH antibody western blot analysis in Ramos lysate. Predicted molecular weight ~56 kDa.
PHGDH antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
PHGDH antibody flow cytometric analysis of Ramos cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PHGDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
3-Phosphoglycerate dehydrogenase (PHGDH) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 249-277 from the human protein was used as the immunogen for this PHGDH antibody.
Limitation:
This PHGDH antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Primate
Uniprot #:
O43175