Phospholamban Antibody / PLN

NSJ Bioreagents
Product Code: NSJ-F51974
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51974-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51974-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Phospholamban antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Phospholamban antibody flow cytometric analysis of Jurkat cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Phospholamban antibody IHC analysis in formalin fixed and paraffin embedded mouse heart tissue.
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Western blot analysis of PLB / Phospholamban antibody and Jurkat lysate

Phospholamban antibody flow cytometric analysis of Jurkat cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Phospholamban antibody IHC analysis in formalin fixed and paraffin embedded mouse heart tissue.
Western blot analysis of PLB / Phospholamban antibody and Jurkat lysate

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the Phospholamban antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the Phospholamban antibody.
Limitation:
This Phospholamban antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P26678