PKM2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F51508
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51508-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51508-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PKM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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PKM2 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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PKM2 antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma.
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Western blot analysis of PKM2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (2). Predicted molecular weight ~ 60 kDa.
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PKM2 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
PKM2 antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma.
Western blot analysis of PKM2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (2). Predicted molecular weight ~ 60 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PKM2 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 116-145 from the human protein was used as the immunogen for this PKM2 antibody.
Limitation:
This PKM2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rabbit, Rat
Uniprot #:
P14618