Retinoblastoma Antibody

NSJ Bioreagents
Product Code: NSJ-F51209
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51209-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51209-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Retinoblastoma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of Retinoblastoma antibody and 293 lysate
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IHC analysis of FFPE human testis tissue stained with Retinoblastoma antibody
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Retinoblastoma antibody flow cytometric analysis of 293 cells (green) compared to a <a href=../tds/rabbit-igg-isotype-control-polyclonal-antibody-n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of Retinoblastoma antibody and 293 lysate
IHC analysis of FFPE human testis tissue stained with Retinoblastoma antibody
Retinoblastoma antibody flow cytometric analysis of 293 cells (green) compared to a <a href=../tds/rabbit-igg-isotype-control-polyclonal-antibody-n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the Retinoblastoma antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
RB1/Retinoblastoma is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 858-886 from the human protein was used as the immunogen for this Retinoblastoma antibody.
Limitation:
This Retinoblastoma antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Rat
Uniprot #:
P06400