SPARC Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F49838
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49838-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49838-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SPARC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot analysis of SPARC antibody and Y79 lysate. Predicted molecular weight 35-45 kDa depending on glycosylation level
2 / 4
Western blot analysis of SPARC antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the SPARC gene.
3 / 4
IHC analysis of FFPE human skin tissue stained with SPARC antibody
4 / 4
Flow cytometric analysis of WiDr cells using SPARC antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
❮❯

Western blot analysis of SPARC antibody and Y79 lysate. Predicted molecular weight 35-45 kDa depending on glycosylation level
Western blot analysis of SPARC antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the SPARC gene.
IHC analysis of FFPE human skin tissue stained with SPARC antibody
Flow cytometric analysis of WiDr cells using SPARC antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the SPARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 224-251 from the human protein was used as the immunogen for this SPARC antibody.
Limitation:
This SPARC antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Pig, Chicken, Rabbit, Xenopus
Uniprot #:
P09486