XBP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F49014
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49014-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49014-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the XBP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of XBP1 antibody and Ramos lysate. Predicted molecular weight: ~29 kDa (isoform 1) and ~40 kDa (isoform 2).
2 / 4
Western blot analysis of XBP1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene.
3 / 4
XBP1 antibody flow cytometric analysis of Ramos cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
XBP1 antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue.
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Western blot analysis of XBP1 antibody and Ramos lysate. Predicted molecular weight: ~29 kDa (isoform 1) and ~40 kDa (isoform 2).
Western blot analysis of XBP1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene.
XBP1 antibody flow cytometric analysis of Ramos cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
XBP1 antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the XBP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 62-91 from the human protein was used as the immunogen for this XBP1 antibody.
Limitation:
This XBP1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine
Uniprot #:
P17861