VIM Antibody

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NSJ Bioreagents
Product Code: NSJ-F48163
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48163-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48163-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For long term storage, aliquot the VIM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of VIM antibody and A2058, A375 lysate.
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Western blot analysis of VIM antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2).
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IHC analysis of FFPE human breast carcinoma tissue stained with VIM antibody
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VIM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue.
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VIM antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC secondary Ab was used.
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Western blot analysis of VIM antibody and A2058, A375 lysate.
Western blot analysis of VIM antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2).
IHC analysis of FFPE human breast carcinoma tissue stained with VIM antibody
VIM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue.
VIM antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC secondary Ab was used.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the VIM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 152-181 from the human protein was used as the immunogen for this VIM antibody.
Limitation:
This VIM antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Hamster, Mouse, Pig, Rat
Uniprot #:
P08670