CD14 Antibody

NSJ Bioreagents
Product Code: NSJ-F49569
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49569-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49569-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the CD14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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CD14 antibody western blot analysis in A549 lysate. Predicted size 40-55 kDa depending on glycosylation level
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Western blot analysis of CD14 antibody and untransfected/transfected 293 cell lysate
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CD14 antibody used to stain FFPE human lung carcinoma
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CD14 antibody flow cytometric analysis of A549 cells (green) compared to a negative control (blue).

CD14 antibody western blot analysis in A549 lysate. Predicted size 40-55 kDa depending on glycosylation level
Western blot analysis of CD14 antibody and untransfected/transfected 293 cell lysate
CD14 antibody used to stain FFPE human lung carcinoma
CD14 antibody flow cytometric analysis of A549 cells (green) compared to a negative control (blue).

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
In concert with LBP, CD14 binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 292-322 from the human protein was used as the immunogen for this CD14 antibody.
Limitation:
This CD14 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P08571