IL-1 Antibody

NSJ Bioreagents
Product Code: NSJ-F49920
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49920-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49920-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the IL-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IL-1 antibody flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
2 / 3
IHC analysis of FFPE human lung carcinoma stained with IL-1 antibody
3 / 3
Western blot analysis of IL-1 antibody and HepG2 lysate. Predicted molecular weight of IL1A: 31 kDa precursor and 17 kDa mature.

IL-1 antibody flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human lung carcinoma stained with IL-1 antibody
Western blot analysis of IL-1 antibody and HepG2 lysate. Predicted molecular weight of IL1A: 31 kDa precursor and 17 kDa mature.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the IL-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IL1A is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 177-206 from human IL1A was used as the immunogen for this IL-1 antibody.
Limitation:
This IL-1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P01583