NKX3.1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F51471
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51471-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51471-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the NKX3.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human prostate carcinoma stained with NKX3.1 antibody
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Western blot analysis of NKX3.1 antibody and MCF-7 lysate. Predicted molecular weight ~28 kDa, observed at 28-38 kDa.
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NKX3.1 antibody flow cytometry analysis of MDA-MB231 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IHC analysis of FFPE human prostate carcinoma stained with NKX3.1 antibody
Western blot analysis of NKX3.1 antibody and MCF-7 lysate. Predicted molecular weight ~28 kDa, observed at 28-38 kDa.
NKX3.1 antibody flow cytometry analysis of MDA-MB231 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the NKX3.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NKX3.1 is a transcription factor which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 118-145 from the human protein was used as the immunogen for this NKX3.1 antibody.
Limitation:
This NKX3.1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
Q99801