CD1e Antibody

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NSJ Bioreagents
Product Code: NSJ-F52013
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52013-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52013-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the CD1e antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of CD1e antibody with MDA-MB435 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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CD1e antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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CD1e antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen.
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CD1e antibody western blot analysis in MDA-MB435 lysate
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Confocal immunofluorescent analysis of CD1e antibody with MDA-MB435 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
CD1e antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
CD1e antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen.
CD1e antibody western blot analysis in MDA-MB435 lysate

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the CD1e antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD1E encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 184-212 from the human protein was used as the immunogen for this CD1e antibody.
Limitation:
This CD1e antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P15812