FGFR2 Antibody

NSJ Bioreagents
Product Code: NSJ-F50618
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50618-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50618-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the FGFR2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Confocal immunofluorescent analysis of FGFR2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
2 / 4
IHC analysis of FFPE human hepatocarcinoma tissue stained with the FGFR2 antibody
3 / 4
FGFR2 antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
Western blot analysis of FGFR2 antibody and 293 lysate transiently transfected with the human gene. Predicted molecular weight: 80-120 kDa

Confocal immunofluorescent analysis of FGFR2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human hepatocarcinoma tissue stained with the FGFR2 antibody
FGFR2 antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of FGFR2 antibody and 293 lysate transiently transfected with the human gene. Predicted molecular weight: 80-120 kDa

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the FGFR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Fibroblast growth factor receptor 2 is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 794-821 from the human protein was used as the immunogen for this FGFR2 antibody.
Limitation:
This FGFR2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
P21802