SOD1 Antibody

NSJ Bioreagents
Product Code: NSJ-F51318
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51318-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51318-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SOD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human colon carcinoma stained with SOD1 antibody
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Western blot analysis of SOD1 antibody and Jurkat lysate.
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SOD1 antibody flow cytometric analysis of Jurkat cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Confocal immunofluorescent analysis of SOD1 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

IHC analysis of FFPE human colon carcinoma stained with SOD1 antibody
Western blot analysis of SOD1 antibody and Jurkat lysate.
SOD1 antibody flow cytometric analysis of Jurkat cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of SOD1 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the SOD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 55-84 from the human protein was used as the immunogen for this SOD1 antibody.
Limitation:
This SOD1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P00441