Src Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F52054
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52054-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52054-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Src antibody western blot analysis in (1) MCF-7 and (2) A431 lysate
2 / 6
Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue
3 / 6
Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue
4 / 6
Src antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary Ab was used for the analysis.
5 / 6
Confocal immunofluorescent analysis of Src antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
6 / 6
Confocal immunofluorescent analysis of Src antibody with A431 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
❮❯

Src antibody western blot analysis in (1) MCF-7 and (2) A431 lysate
Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue
Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue
Src antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control (left histogram). Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of Src antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of Src antibody with A431 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:2000,IHC (Paraffin): 1:50,Flow Cytometry: 1:50,Immunofluorescence: 1:50
Application Note:
Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 24-52 from the human protein was used as the immunogen for this Src antibody.
Limitation:
This Src antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
P12931