SUMO Antibody

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NSJ Bioreagents
Product Code: NSJ-F42009
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42009-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42009-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SUMO antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Fluorescent image of HeLa cells stained with SUMO antibody at 1:25. SUMO1 immunoreactivity is localized to the nucleus.
2 / 4
SUMO antibody flow cytometric analysis of HeLa cells (right histogram) compared to a?<a href=../search_result.php?search_txt=n1001>negative control</a>?(left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 4
IHC analysis of FFPE human breast carcinoma tissue stained with the SUMO antibody
4 / 4
Western blot analysis of SUMO antibody and A375 lysate. Predicted molecular weight: 12-15 kDa
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Fluorescent image of HeLa cells stained with SUMO antibody at 1:25. SUMO1 immunoreactivity is localized to the nucleus.
SUMO antibody flow cytometric analysis of HeLa cells (right histogram) compared to a?<a href=../search_result.php?search_txt=n1001>negative control</a>?(left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human breast carcinoma tissue stained with the SUMO antibody
Western blot analysis of SUMO antibody and A375 lysate. Predicted molecular weight: 12-15 kDa

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the SUMO antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 52-79 from the human protein was used as the immunogen for this SUMO antibody.
Limitation:
This SUMO antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P63165