SUMO1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F42001
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42001-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42001-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SUMO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of SUMO1 antibody with A375 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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SUMO1 antibody flow cytometric analysis of HeLa cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue).
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IHC analysis of FFPE human breast carcinoma tissue stained with the SUMO1 antibodyFITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Confocal immunofluorescent analysis of SUMO1 antibody with A375 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
SUMO1 antibody flow cytometric analysis of HeLa cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue).
IHC analysis of FFPE human breast carcinoma tissue stained with the SUMO1 antibodyFITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the SUMO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for this SUMO1 antibody.
Limitation:
This SUMO1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Pig
Uniprot #:
P63165