AFP Antibody

NSJ Bioreagents
Product Code: NSJ-F43722
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F43722-0.08ML0.08 ml£260.00
Quantity:
NSJ-F43722-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the AFP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human hepatocarcinoma tissue stained with AFP antibody~
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Western blot analysis of AFP antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the AFP gene (2). Predicted molecular

IHC analysis of FFPE human hepatocarcinoma tissue stained with AFP antibody~
Western blot analysis of AFP antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the AFP gene (2). Predicted molecular

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the AFP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 300-329 from the human protein was used as the immunogen for this AFP antibody.
Limitation:
This AFP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P02771