NSJ Bioreagents

Dnmt3b Antibody

Product Code:
 
NSJ-F40611
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the Dnmt3b antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of Dnmt3b antibody at 1:2000 dilution + T47D lysate; Predicted molecular weight: 95 kDa.
2 / 3
Western blot testing of Dnmt3b antibody at 1:2000 dilution. Lane 1: A431 lysate; 2: A549; Predicted molecular weight: 95 kDa.
3 / 3
IHC analysis of FFPE human prostate carcinoma tissue stained with Dnmt3b antibody
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Western blot testing of Dnmt3b antibody at 1:2000 dilution + T47D lysate; Predicted molecular weight: 95 kDa.
Western blot testing of Dnmt3b antibody at 1:2000 dilution. Lane 1: A431 lysate; 2: A549; Predicted molecular weight: 95 kDa.
IHC analysis of FFPE human prostate carcinoma tissue stained with Dnmt3b antibody

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CodeSizePrice
NSJ-F40611-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40611-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:2000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the Dnmt3b antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 389-417 from the human protein was used as the immunogen for this Dnmt3b antibody.
Limitation:
This Dnmt3b antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q9UBC3