NSJ Bioreagents

EIF4E Antibody

Product Code:
 
NSJ-F47410
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the EIF4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 2
Western blot analysis of EIF4E antibody and HL-60 lysate. Predicted molecular weight ~27 kDa.~
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IHC analysis of FFPE human breast carcinoma stained with EIF4E antibody
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Western blot analysis of EIF4E antibody and HL-60 lysate. Predicted molecular weight ~27 kDa.~
IHC analysis of FFPE human breast carcinoma stained with EIF4E antibody

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CodeSizePrice
NSJ-F47410-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47410-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 32-61 from the human protein was used as the immunogen for this EIF4E antibody.
Limitation:
This EIF4E antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Zebrafish, Rabbit, Xenopus
Uniprot #:
P06730