Epidermal growth factor receptor Antibody

Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Titration of the Epidermal growth factor receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. [UniProt]

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

This EGFR antibody was produced from rabbits immunized with EGFR his fusion protein.

This Epidermal growth factor receptor antibody is available for research use only.

Purified

Human

P00533