Phospho-KDR Antibody (pY996)

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NSJ Bioreagents
Product Code: NSJ-F48399
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48399-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48399-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the phospho-KDR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Phospho-KDR antibody used in western blot to detect phosphorylated KDR/FLK1 in HMVEC lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min and Ab used at 1:500. Predicted molecular weight ~ 152 kDa.~
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IHC analysis of FFPE
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Phospho-KDR antibody used in western blot to detect phosphorylated KDR/FLK1 in HMVEC lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min and Ab used at 1:500. Predicted molecular weight ~ 152 kDa.~
IHC analysis of FFPE

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the phospho-KDR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-KDR antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY996 of human KDR/FLK1.
Limitation:
This phospho-KDR antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P35968