SPHK2 Antibody

Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Titration of the SPHK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 1-30 from the human protein was used as the immunogen for this SPHK2 antibody.

This SPHK2 antibody is available for research use only.

Purified

Human

Q9NRA0