UBC9 Antibody

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NSJ Bioreagents
Product Code: NSJ-F40817
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40817-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40817-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the UBC9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of UBE2I antibody and A2058 lysate.
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Western blot analysis of UBE2I antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the UBE2I gene.
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IHC analysis of FFPE human breast carcinoma tissue stained with the UBE2I antibody
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Western blot analysis of UBE2I antibody and A2058 lysate.
Western blot analysis of UBE2I antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the UBE2I gene.
IHC analysis of FFPE human breast carcinoma tissue stained with the UBE2I antibody

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the UBC9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for this UBC9 antibody.
Limitation:
This UBC9 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Chicken, Zebrafish, Xenopus
Uniprot #:
P63279