UCHL3 Antibody

Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Titration of the UCHL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 195-225 from the human protein was used as the immunogen for this UCHL3 antibody.

This UCHL3 antibody is available for research use only.

Purified

Human

Mouse, Rat, Bovine, Pig

P15374