Anti-Ubiquitin Antibody

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NSJ Bioreagents
Product Code: NSJ-F42055
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42055-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42055-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Can be stored at 4oC for one month. For long-term, aliquot the anti-Ubiquitin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human breast carcinoma with anti-Ubiquitin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human kidney tissue with anti-Ubiquitin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of 1) human 293, 2) human HeLa, 3) human HepG2, 4) human A549, 5) mouse NIH 3T3, 6) rat C2C12 and 7) rat C6 cell lysate with anti-Ubiquitin antibody. Predicted molecular weight ~8 kDa.
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IHC staining of FFPE human breast carcinoma with anti-Ubiquitin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with anti-Ubiquitin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human 293, 2) human HeLa, 3) human HepG2, 4) human A549, 5) mouse NIH 3T3, 6) rat C2C12 and 7) rat C6 cell lysate with anti-Ubiquitin antibody. Predicted molecular weight ~8 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the anti-Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. It is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-32 from the human protein was used as the immunogen for this anti-Ubiquitin antibody.
Limitation:
This anti-Ubiquitin antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Pig, Primate, Chicken, Hamster, Xenopus, Drosophila
Uniprot #:
P0CG48