ALDH1A1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F44049
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44049-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44049-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ALDH1A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of ALDH1A1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (2).
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IHC analysis of FFPE human hepatocarcinoma tissue stained with ALDH1A1 antibody
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Immunofluorescence analysis of anti-ALDH1A1 antibody and HeLa cells. Primary Ab was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
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Flow cytometric analysis of HepG2 cells using ALDH1A1 antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of ALDH1A1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (2).
IHC analysis of FFPE human hepatocarcinoma tissue stained with ALDH1A1 antibody
Immunofluorescence analysis of anti-ALDH1A1 antibody and HeLa cells. Primary Ab was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow cytometric analysis of HepG2 cells using ALDH1A1 antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the ALDH1A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ALDH1A1 binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity). [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 302-331 from the human protein was used as the immunogen for this ALDH1A1 antibody.
Limitation:
This ALDH1A1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Primate
Uniprot #:
P00352