Anti-PCNA Antibody

NSJ Bioreagents
Product Code: NSJ-F48250
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48250-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48250-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the anti-PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of anti-PCNA antibody and Jurkat (lane 1), HeLa (2), 293 (3) lysate
2 / 4
IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-PCNA antibody
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Confocal immunofluorescent analysis of anti-PCNA antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
4 / 4
Anti-PCNA antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of anti-PCNA antibody and Jurkat (lane 1), HeLa (2), 293 (3) lysate
IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-PCNA antibody
Confocal immunofluorescent analysis of anti-PCNA antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Anti-PCNA antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the anti-PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 89-117 from the human protein was used as the immunogen for this anti-PCNA antibody.
Limitation:
This anti-PCNA antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Primate, Chicken, Hamster
Uniprot #:
P12004